AHR Antibody

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ProSci
Product Code: PSI-27-379
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-379-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AHR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
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Antibody used in WB on Human HepG2 at: 1:1000 (Lane1: 50 ug HepG2 nuclei extract + benzo[a]pyrene, Lane2: 50 ug HepG2 cytoplasm extract + benzo[a]pyrene).
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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human HepG2 at: 1:1000 (Lane1: 50 ug HepG2 nuclei extract + benzo[a]pyrene, Lane2: 50 ug HepG2 cytoplasm extract + benzo[a]pyrene).

Documents

Further Information

Additional Names:
AHR, bHLHe76
Application Note:
AHR antibody can be used for detection of AHR by ELISA at 1:12500. AHR antibody can be used for detection of AHR by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. AHR ligands included a variety of aromatic hydrocarbons.
Background References:
  • Marlowe, J.L., et al., (2004) J. Biol. Chem. 279 (28), 29013-29022.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AHR.
NCBI Gene ID #:
196
NCBI Official Name:
aryl hydrocarbon receptor
NCBI Official Symbol:
AHR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
96 kDa
Protein Accession #:
NP_001612
Protein GI Number:
4502003
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cell Cycle ,Cancer ,Signal Transduction ,Primary ///Apoptosis ,
Swissprot #:
P35869
User NOte:
Optimal dilutions for each application to be determined by the researcher.