Transcription Factor A Antibody

ProSci
Product Code: PSI-27-334
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-334-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Transcription Factor A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
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Antibody used in WB on Mouse bEND.3 at lane1:10 ug and lane 2:20 ug. (lane1: 10 ug , lane 2:20 ug.).
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Antibody used in WB on Human cell lines at: 1:1000 (Lane 1 and 2: HepG2 cell lysate, Lane 3 and 4: Hep3B cell lysate, Lane 5 and 6: K562 cell lysate).

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in WB on Mouse bEND.3 at lane1:10 ug and lane 2:20 ug. (lane1: 10 ug , lane 2:20 ug.).
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1 and 2: HepG2 cell lysate, Lane 3 and 4: Hep3B cell lysate, Lane 5 and 6: K562 cell lysate).

Further Information

Additional Names:
Transcription Factor AntibodiesAM, TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3
Application Note:
Transcription Factor A antibody can be used for detection of Transcription Factor A by ELISA at 1:1562500. Transcription Factor A antibody can be used for detection of Transcription Factor A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The function remains unknown.This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Background References:
  • Eve A. Roberts, et al., (2007) Molecular Genetics Metabolism.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Transcription Factor A.
NCBI Gene ID #:
7019
NCBI Official Name:
transcription factor A, mitochondrial
NCBI Official Symbol:
TFAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
NP_003192
Protein GI Number:
4507401
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cell Cycle ,Signal Transduction
Swissprot #:
Q00059
User NOte:
Optimal dilutions for each application to be determined by the researcher.