TRPC6 Antibody
| Code | Size | Price |
|---|
| PSI-46-530-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TRPC6, transient receptor potential cation channel, subfamily C, member 6, HGNC:12338, TRP6, transient receptor potential channel 6, FLJ11098, FLJ14863, FSGS2, TRP6
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 100kDa band observed in lysates of cell line A549 (calculated MW of 106kDa according to NP_004612.2). Preliminary experiments also showed 100kDa in Human Lung lysates and in lysates of cell line HEK293 .The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Background References:
- Winn MP, Conlon PJ, Lynn KL, Farrington MK, Creazzo T, Hawkins AF, Daskalakis N, Kwan SY, Ebersviller S, Burchette JL, Pericak-Vance MA, Howell DN, Vance JM, Rosenberg PB. A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis. Science. 2005 Jun 17;308(5729):1801-4. Epub 2005 May 5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EKLSMEPNQEETN
NCBI Gene ID #:
7225
NCBI Official Name:
transient receptor potential cation channel, subfamily C, member 6
NCBI Official Symbol:
TRPC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004612.2
Protein GI Number:
5730102
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9Y210