POLR3B Antibody
| Code | Size | Price |
|---|
| PSI-30-455-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Fly
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
POLR3B, FLJ10388, RPC2, C128, HLD8
Application Note:
POLR3B antibody can be used for detection of POLR3B by ELISA at 1:12500. POLR3B antibody can be used for detection of POLR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Background References:
- Yee, N.S., PLoS Biol. 5 (11), E312 (2007).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3B.
NCBI Gene ID #:
55703
NCBI Official Name:
polymerase (RNA) III (DNA directed) polypeptide B
NCBI Official Symbol:
POLR3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
128 kDa
Protein Accession #:
NP_060552
Protein GI Number:
238908503
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q9NW08
User NOte:
Optimal dilutions for each application to be determined by the researcher.