cTnI Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-141-0.1mL | 0.1mL | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3A10A12
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
cTnI monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
TNNC1, Cardiac troponin ICMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF
Application Note:
Western Blot:Dilution 1:500 - 1:2,000
IHC(P):Dilution 1:500 - 2,000
IHC(F):Dilution 1:500 - 2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):Dilution 1:500 - 2,000
IHC(F):Dilution 1:500 - 2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
Background References:
- Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010.
- Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
- Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
- Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
7137
NCBI Official Name:
troponin I type 3 (cardiac)
NCBI Official Symbol:
TNNI3
NCBI Organism:
Homo sapiens
Protein Accession #:
P19429
Protein GI Number:
136213
Swissprot #:
P19429
User NOte:
Optimal dilutions for each application to be determined by the researcher.