P16 Monoclonal Antibody

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ProSci
Product Code: PSI-32-202
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-202-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2D9A12
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
P16 (Mouse and Human) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.
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Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.

Documents

Further Information

Additional Names:
ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Background:
The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.
Background References:
  • Hunter, T. 1993. Cell 75: 839-841.
  • Sherr, C.J. 1993. Cell 73: 1059-1065.
  • El-Deiry, W.S., et al. 1993. Cell 75: 817-825.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
1029
NCBI Official Name:
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol:
CDKN2A
NCBI Organism:
Homo sapiens
Protein Accession #:
NP_000068.1
Protein GI Number:
4502749
Swissprot #:
P42771
User NOte:
Optimal dilutions for each application to be determined by the researcher.