HMHA1 Antibody

ProSci
Product Code: PSI-42-988
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-988-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-988 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-988 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
HMHA1, histocompatibility (minor) HA-1, HA-1, HLA-HA1, KIAA0223, minor histocompatibility antigen HA-1, minor histocompatibility protein HA-1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 200kDa band in lysates of cell lines Jurkat and Molt4 after 0.05ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kDa according to NP_036424.2. The 200kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: KATGTLKRPTSLSRH-C
NCBI Gene ID #:
23526
NCBI Official Name:
histocompatibility (minor) HA-1
NCBI Official Symbol:
HMHA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036424.2
Protein GI Number:
47834348
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)
Swissprot #:
Q92619