LGP2 Antibody

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ProSci
Product Code: PSI-46-863
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-863-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-863 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-863 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
DHX58, LGP2, DEXH (Asp-Glu-X-His) box polypeptide 58 , D11LGP2 , D11lgp2e , LGP2, RNA helicase LGP2 , ortholog of mouse D11lgp2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 80kDa band observed in Human Liver lysates (calculated MW of 76.6kDa according to NP_077024.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 24kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Saito T, Hirai R, Loo YM, Owen D, Johnson CL, Sinha SC, Akira S, Fujita T, Gale M Jr. Regulation of innate antiviral defenses through a shared repressor domain in RIG-I and LGP2. Proc Natl Acad Sci USA. 2007 Jan 9;104(2):582-7. Epub 2006 Dec 26.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: DFLQHCAENLSD
NCBI Gene ID #:
79132
NCBI Official Name:
DEXH (Asp-Glu-X-His) box polypeptide 58
NCBI Official Symbol:
DHX58
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_077024.2
Protein GI Number:
149408122
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
.
Swissprot #:
Q96C10