MARCH6 Antibody

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ProSci
Product Code: PSI-46-718
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-718-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-718 (0.2ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-718 (0.2ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MARCH-VI, TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, membrane-associated RING-CH protein VI
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 100kDa band observed in Human Lung lysates (calculated MW of 103kDa according to NP_005876.2). Recommended concentration: 0.2-0.5ug/ml. An additional band of unknown identity was also consistently observed at 18kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Kreft SG, Wang L, Hochstrasser M. Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem. 2006 Feb 24;281(8):4646-53. Epub 2005 Dec 22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-QGRRVIFQKVKE
NCBI Gene ID #:
10299
NCBI Official Name:
membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase
NCBI Official Symbol:
Mar-06
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005876.2
Protein GI Number:
33589846
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
O60337