VHNF1 Antibody
| Code | Size | Price |
|---|
| PSI-46-572-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide.Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.
Background References:
- Terasawa K, Toyota M, Sagae S, Ogi K, Suzuki H, Sonoda T, Akino K, Maruyama R, Nishikawa N, Imai K, Shinomura Y, Saito T, Tokino T. Epigenetic inactivation of TCF2 in ovarian cancer and various cancer cell lines. Br J Cancer. 2006 Mar 27;94(6):914-21.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-QAYDRQKNPSKEER
NCBI Gene ID #:
6928
NCBI Official Name:
HNF1 homeobox B
NCBI Official Symbol:
HNF1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000449.1, NP_001159395.1
Protein GI Number:
4507397
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.
Swissprot #:
P35680