SPI1 Antibody
| Code | Size | Price |
|---|
| PSI-25-419-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A
Application Note:
SPI1 antibody can be used for detection of SPI1 by ELISA at 1:312500. SPI1 antibody can be used for detection of SPI1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SPI1 is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. Lack of SPI1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
- Crotti, T.N., (2008) J. Cell. Physiol. 215 (3), 636-644.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPI1.
NCBI Gene ID #:
6688
NCBI Official Name:
spleen focus forming virus (SFFV) proviral integration oncogene
NCBI Official Symbol:
SPI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
NP_003111
Protein GI Number:
124028517
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,RNA Binding ,Cancer
Swissprot #:
P17947
User NOte:
Optimal dilutions for each application to be determined by the researcher.