ILF2 Antibody

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ProSci
Product Code: PSI-28-339
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-28-339-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ILF2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in IHC on Human kidney.
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Antibody used in WB on Human MCF-7 at 1.5mg/mL.
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Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
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Antibody used in IHC on Human kidney.
Antibody used in WB on Human MCF-7 at 1.5mg/mL.
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).

Documents

Further Information

Additional Names:
ILF2, NF45, PRO3063
Application Note:
ILF2 antibody can be used for detection of ILF2 by ELISA at 1:62500. ILF2 antibody can be used for detection of ILF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
Background References:
  • Warner C. Greene, et al., (2006) Proc Natl Acad Sci 103:15588.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF2.
NCBI Gene ID #:
3608
NCBI Official Name:
interleukin enhancer binding factor 2
NCBI Official Symbol:
ILF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_004506
Protein GI Number:
24234747
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,RNA Binding ,Signal Transduction ,Immunology
Swissprot #:
Q12905
User NOte:
Optimal dilutions for each application to be determined by the researcher.