NFX1 Antibody
| Code | Size | Price |
|---|
| PSI-25-227-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
NFX1, DKFZp779G2416, MGC20369, NFX2, Tex42, TEG-42
Application Note:
NFX1 antibody can be used for detection of NFX1 by ELISA at 1:62500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
NFX1 binds to the X-box motif of MHC class II genes and represses their expression. NFX1 may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. NFX1 mediates E2-dependent ubiquitination.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Background References:
- Katzenellenbogen, R.A., (2007) J. Virol. 81 (8), 3786-3796.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFX1.
NCBI Gene ID #:
4799
NCBI Official Name:
nuclear transcription factor, X-box binding 1
NCBI Official Symbol:
NFX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
113 kDa
Protein Accession #:
NP_667344
Protein GI Number:
22212925
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Signal Transduction ,Immunology
Swissprot #:
Q12986-2
User NOte:
Optimal dilutions for each application to be determined by the researcher.