VPS25 Antibody

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ProSci
Product Code: PSI-46-578
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-578-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-578 (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-578 (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), FAP20
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Vaccari T, Bilder D. The Drosophila Tumor Suppressor vps25 Prevents Nonautonomous Overproliferation by Regulating Notch Trafficking. Dev Cell. 2005 Nov;9(5):687-98.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-QPNVDTRQKQ
NCBI Gene ID #:
84313
NCBI Official Name:
vacuolar protein sorting 25 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115729.1
Protein GI Number:
14150155
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9BRG1