AFX Antibody

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ProSci
Product Code: PSI-79-168
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-168-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197).

Documents

Further Information

Additional Names:
AFX, AFX1, MLLT7, EIF2A, AFX, FOXO4, Afxh
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Background References:
  • Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21 (10): 3534-46. Kops GJ, et al. (1999) Nature; 398 (6728): 630-4.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4303
NCBI Official Name:
forkhead box O4
NCBI Official Symbol:
FOXO4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
NP_001164402.1
Protein GI Number:
283436083
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of AFX only when phosphorylated at serine 197.
Swissprot #:
P98177