GTF2E1 Antibody
| Code | Size | Price |
|---|
| PSI-31-216-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2E1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
GTF2E1, FE, TF2E1, TFIIE-A
Application Note:
GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
Background References:
- Ohkuma, Y., et al., (1991) Nature 354 (6352), 398-401.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.
NCBI Gene ID #:
2960
NCBI Official Name:
general transcription factor IIE, polypeptide 1, alpha 56kDa
NCBI Official Symbol:
GTF2E1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_005504
Protein GI Number:
144922725
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P29083
User NOte:
Optimal dilutions for each application to be determined by the researcher.