POLR3F Antibody

ProSci
Product Code: PSI-26-338
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-26-338-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR3F antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
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Antibody used in WB on Human 721_B at 0.2-1 ug/ml.

Further Information

Additional Names:
POLR3F, MGC13517, RPC39, RPC6
Application Note:
POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.
Background References:
  • Stelzl, U., (2005) Cell 122 (6), 957-968.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3F.
NCBI Gene ID #:
10621
NCBI Official Name:
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
NCBI Official Symbol:
POLR3F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
NP_006457
Protein GI Number:
33598952
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q9H1D9
User NOte:
Optimal dilutions for each application to be determined by the researcher.