TRMT5 Antibody

ProSci
Product Code: PSI-26-357
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-26-357-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRMT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

Further Information

Additional Names:
TRMT5, KIAA1393, MGC111453, TRM5
Application Note:
TRMT5 antibody can be used for detection of TRMT5 by ELISA at 1:62500. TRMT5 antibody can be used for detection of TRMT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).
Background References:
  • Brule, H., (2004) Biochemistry 43 (28), 9243-9255.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRMT5.
NCBI Gene ID #:
57570
NCBI Official Name:
tRNA methyltransferase 5
NCBI Official Symbol:
TRMT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
NP_065861
Protein GI Number:
145275187
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q32P41
User NOte:
Optimal dilutions for each application to be determined by the researcher.