ARF1 Antibody

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ProSci
Product Code: PSI-45-277
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-277-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-277 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-277 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARF1, ARF 1, ADP-ribosylation factor 1, ADP ribosylation factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Background References:
  • Dubois T, Paleotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P. Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics. Nat Cell Biol. 2005 Apr;7(4):353-64. Epub 2005 Mar 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EGLDWLSNQLRNQK
NCBI Gene ID #:
375; 376, 377, 378
NCBI Official Name:
ADP-ribosylation factor 1
NCBI Official Symbol:
ARF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1
Protein GI Number:
66879660
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all four reported isoforms.
Swissprot #:
P84077