CBX1 Antibody

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ProSci
Product Code: PSI-45-370
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-370-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 2
45-370 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
2 / 2
45-370 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-370 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-370 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CBX1, HP1-BETA, chromobox homolog 1 (HP1 beta homolog Drosophila ), HGNC:1551, CBX, HP1Hs-beta, M31, MOD1, chromobox homolog 1 (Drosophila HP1 beta), heterochromatin protein p25 beta, HP1Hsbeta, p25beta, heterochromatin protein 1-beta, modifier 1 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 25kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.
Background References:
  • Sharma GG, Hwang KK, Pandita RK, Gupta A, Dhar S, Parenteau J, Agarwal M, Worman HJ, Wellinger RJ, Pandita TK. Human heterochromatin protein 1 isoforms HP1(Hsalpha) and HP1(Hsbeta) interfere with hTERT-telomere interactions and correlate with changes in cell growth and response to ionizing radiation. Mol Cell Biol. 2003 Nov;23(22):8363-76.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QSQKTAHETDKSE
NCBI Gene ID #:
10951
NCBI Official Name:
chromobox homolog 1
NCBI Official Symbol:
CBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006798.1, NP_001120700.1
Protein GI Number:
5803076
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Variants (NP_006798.1; NP_001120700.1) encode the same protein.
Swissprot #:
P83916