CDK6 Antibody

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ProSci
Product Code: PSI-79-249
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-249-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24).
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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24).

Documents

Further Information

Additional Names:
PLSTIRE, CREB2, CREBP1, Serine/threonine-protein kinase PLSTIRE
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
Background References:
  • Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659. Pestell, R. G. et al. (1999) Endocr. Rev. 20, 501-534. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CDK6 (phospho-Tyr24) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y (p) -G-K) derived from Human CDK6.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1021
NCBI Official Name:
cyclin-dependent kinase 6
NCBI Official Symbol:
CDK6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
NP_001138778.1
Protein GI Number:
223718134
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 24.
Swissprot #:
Q00534