EBAG9 Antibody

ProSci
Product Code: PSI-45-515
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-515-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-515 (0.2ug/ml) staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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45-515 (0.2ug/ml) staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
EBAG9, RCAS1, estrogen receptor binding site associated, antigen, 9 , HGNC:3123, EB9, PDAF, cancer associated surface antigen, estrogen receptor binding site associated antigen 9
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 24.4kDa according to NP_004206.1and NP_004206.1). Recommended concentration: 1-3ug/ml. A minor band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml.
Background References:
  • Ruder C, Reimer T, Delgado-Martinez I, Hermosilla R, Engelsberg A, Nehring R, Dorken B, Rehm A. EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin. Mol Biol Cell. 2005 Mar;16(3):1245-57. Epub 2005 Jan 5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KEAQRLMKKEQN
NCBI Gene ID #:
9166
NCBI Official Name:
estrogen receptor binding site associated, antigen, 9
NCBI Official Symbol:
EBAG9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004206.1
Protein GI Number:
4758230
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O00559