EHD2 Antibody

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ProSci
Product Code: PSI-45-523
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-523-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-523 (0.1ug/ml) staining of A549 (A) and (0.3ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-523 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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45-523 (0.1ug/ml) staining of A549 (A) and (0.3ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-523 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Documents

Further Information

Additional Names:
EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunofluorescence: strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10?g/ml.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Background References:
  • Guilherme A, Soriano NA, Furcinitti PS, Czech MP. Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes. J Biol Chem. 2004 Sep 17;279(38):40062-75. Epub 2004 Jul 09.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CRLVPPSKRRHKGSA
NCBI Gene ID #:
30846
NCBI Official Name:
EH-domain containing 2
NCBI Official Symbol:
EHD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055416.2
Protein GI Number:
21361462
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise EHD1 protein as well as EHD2.
Swissprot #:
Q9NZN4