ELF3 Antibody

ProSci
Product Code: PSI-45-527
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-527-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-527 staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-527 staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Further Information

Additional Names:
ELF3, ERT, E74-like factor 3 (ets domain transcription factor, epithelial-specific ), EPR-1, ESE-1, E74-like factor 3 (ets domain transcription factor), ets domain transcription factor, serine box (epithelial-specific), E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4ug/ml.
Background References:
  • Oettgen P, Alani RM, Barcinski MA, Brown L, Akbarali Y, Boltax J, Kunsch C, Munger K, Libermann TA. Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family. Mol Cell Biol. 1997 Aug;17(8):4419-33.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SGWKEEEVLQSRN
NCBI Gene ID #:
1999
NCBI Official Name:
E74-like factor 3 (ets domain transcription factor, epithelial-specific )
NCBI Official Symbol:
ELF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004424.2
Protein GI Number:
40255035
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P78545