ELMO2 Antibody

ProSci
Product Code: PSI-45-531
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-531-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-531 staining (2ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by chemiluminescence.
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45-531 staining (2ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band seen in Human Brain lysate. Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6ug/ml.
Background References:
  • Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell. 2001 Oct 5;107(1):27-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PKEPSSYDFVYHYG
NCBI Gene ID #:
63916
NCBI Official Name:
engulfment and cell motility 2
NCBI Official Symbol:
ELMO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_877496.1, NP_573403.1
Protein GI Number:
33469947
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
NP_877496.1 and NP_573403.1 are varients that encode the same protein.
Swissprot #:
Q96JJ3