FasL (A11) Monoclonal Antibody

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ProSci
Product Code: PSI-XA-1000
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XA-1000-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rat
Antibody Isotype: Rat IgM
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
Storage:
FasL antibody can be stored at 4˚C, stable for one year.

Images

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The immunogen is a synthetic peptide corresponding to amino acids 196-220 of mouse FasL (CD95L; APO-1L; CD178). The schematic structure of mouse FasL (peptide, aa. 196-220) as seen in this image.
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The immunogen is a synthetic peptide corresponding to amino acids 196-220 of mouse FasL (CD95L; APO-1L; CD178). The schematic structure of mouse FasL (peptide, aa. 196-220) as seen in this image.

Documents

Further Information

Application Note:
FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections).Method: Cryostat sections (6-8 μm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC).
Background References:
  • Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995).
  • Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand: T. Renno, et al.; J. Exp. Med. 183, 431 (1996).
  • Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover: L.E. French, et al.; J. Cell. Biol. 133, 335 (1996).
  • Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FasL (monoclonal A11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
NCBI Gene ID #:
14103
NCBI Official Name:
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol:
Fasl
NCBI Organism:
Mus musculus
Physical State:
Liquid
Purification:
FasL (monoclonal A11) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Apoptosis
Swissprot #:
P41047
User NOte:
Optimal dilutions for each application to be determined by the researcher.