IkB-a Antibody

Contact us
ProSci
Product Code: PSI-79-180
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-180-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

1 / 2
Western blot analysis of extract from HeLa cells untreated or treated with serum using IκB-α (Phospho-Tyr42).
2 / 2
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (phospho-Tyr42).
❮❯

Western blot analysis of extract from HeLa cells untreated or treated with serum using IκB-α (Phospho-Tyr42).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (phospho-Tyr42).

Documents

Further Information

Additional Names:
IKBA, MAD-3, NFKBI, CDC2, CDC28A, CDKN1, P34CDC2, IKBA, NFKBIA,
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Background References:
  • Beraud C, et al. (1999) Proc Natl Acad Sci U S A 96 (2): 429-434. Sundstrom S, et al. (2005) J Virol 79 (4): 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18 (7): 4221-4234. Shrivastava A, et al. (1998) J Virol 72 (12): 9722-9728.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IkB-a (Phospho-Tyr42) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 42 (E-E-Y (p) -E-Q) derived from Human IkappaB-α.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4792
NCBI Official Name:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
NCBI Official Symbol:
NFKBIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
NP_065390.1
Protein GI Number:
10092619
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous levels of IkappaB-α only when phosphorylated at tyrosine 42.
Swissprot #:
P25963