MARCKS Antibody

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ProSci
Product Code: PSI-79-246
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-246-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using MARCKS (phospho-Ser170).
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170).
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Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using MARCKS (phospho-Ser170).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170).

Documents

Further Information

Additional Names:
MACS, 80K-L, PKCSL, PRKCSL, HSP27, HSP28, Protein kinase C substrate, 80 kDa protein, light chain
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Background References:
  • Ramsden, J. J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. Hartwig, J. H. et al. (1992) Nature 356, 618-622. Thelen, M. et al. (1991) Nature 351, 320-322.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MARCKS (phospho-Ser170) antibody was raised against a peptide sequence around phosphorylation site of Serine 170 (G-F-S (p) -F-K) derived from Human MARCKS.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4082
NCBI Official Name:
myristoylated alanine-rich protein kinase C substrate
NCBI Official Symbol:
MARCKS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_002347.5
Protein GI Number:
153070260
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.
Swissprot #:
P29966