MKK6 Antibody

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ProSci
Product Code: PSI-79-172
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-172-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using MKK6 (Phospho-Ser207).
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Western blot analysis of lysed extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207).
<b>Top Image:</b> Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using MKK6 (Phospho-Ser207).

Documents

Further Information

Additional Names:
MEK6, MKK6, MAPKK6, PRKMK6, SAPKK3, SAPKK-3, MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MEK6
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Background References:
  • Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MKK6 (Phospho-Ser207) antibody was raised against a peptide sequence around phosphorylation site of serine 207 (V-D-S (p) -V-A) derived from Human MKK6.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5608
NCBI Official Name:
mitogen-activated protein kinase kinase 6
NCBI Official Symbol:
MAP2K6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
NP_002749.2
Protein GI Number:
14589900
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of MKK6 only when phosphorylated at serine207.
Swissprot #:
P52564