NIPP1 Antibody
| Code | Size | Price |
|---|
| PSI-45-968-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory subunit-8, ARD-1, RNase E, protein phosphatase 1 regulatory inhibitor subunit 8, protein phosphatase 1 regulatory subunit 8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.Immunohistochemistry:In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Beullens M, Bollen M. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem. 2002 May 31;277(22):19855-60. Epub 2002 Mar 21.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EAWPGKKPTPSLLI
NCBI Gene ID #:
5511
NCBI Official Name:
protein phosphatase 1, regulatory subunit 8
NCBI Official Symbol:
PPP1R8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002704.1, NP_054829.2, NP_612568.1
Protein GI Number:
4506015
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all three human isoforms of this protein.
Swissprot #:
Q12972