Perforin (CE2.10) Monoclonal Antibody

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ProSci
Product Code: PSI-XA-1009
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XA-1009-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Perforin antibody can be stored at 4˚C, stable for one year.

Images

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The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.
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The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.

Documents

Further Information

Application Note:
Perforin antibody can be used for detection of horse Perforin by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apoPerforin. Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Background References:
  • Perforin and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997).
  • Perforin: can't live with it-can't live without it:J.C. Reed; Cell 91, 559 (1997).
  • Prevention of apoptosis by Bcl-2: release of Perforin from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997).
  • Apaf-1, a human protein homologous to C. elegans CED-4, participates in Perforin-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Perforin (monoclonal CE2.10) antibody was raised against recombinant mouse perforin (aa. 98-534).
NCBI Gene ID #:
5551
NCBI Official Name:
perforin 1 (pore forming protein)
NCBI Official Symbol:
PRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Perforin (monoclonal CE2.10) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Immunology
Swissprot #:
P14222
User NOte:
Optimal dilutions for each application to be determined by the researcher.