RNF7 Antibody
| Code | Size | Price |
|---|
| PSI-42-275-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
RNF7, ring finger protein 7, CKBBP1, ROC2, SAG, OTTHUMP00000180645, regulator of cullins 2, sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2, zinc RING finger protein SAG
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3ug/ml.
An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Background References:
- Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA, E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Molecular cell 2009 Feb 33 (4): 483-95.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: DACLRCQAENKQE
NCBI Gene ID #:
9616
NCBI Official Name:
ring finger protein 7
NCBI Official Symbol:
RNF7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055060.1
Protein GI Number:
7657522
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_055060.1).
Swissprot #:
Q9UBF6