Ubiquitin Hydrolase 1 Monoclonal Antibody

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ProSci
Product Code: PSI-50-263
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-263-0.1mL0.1mL£686.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Ubiquitin Hydrolase 1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. <br><br> <b>Below:</b> Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.
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Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 24k UCHL1 protein. <br><br> <b>Below:</b> Rat spinal cord stained with anti-UCHL1(red) and anti-neurofilament NF-H antibody (green). The large cells are a-motorneurons and UCHL1 fills the cytoplasm of their perikaryaand dendrites.

Documents

Further Information

Background:
Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker.
Background References:
  • Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983).
  • Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989).
  • Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 10:1963-70 (2001).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant full length human UCHL1 purified from E. coli.
NCBI Gene ID #:
7345
NCBI Official Name:
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol:
UCHL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24
Protein Accession #:
P09936
Protein GI Number:
136681
Purification:
Total IgG fraction
Research Area:
Autophagy
SPECIFICITY:
Specific for the ~24kDa UCHL1 protein.
Swissprot #:
P09936
User NOte:
Optimal dilutions for each application to be determined by the researcher.