Arachidonate 5-lipoxygenase Antibody

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ProSci
Product Code: PSI-45-274
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-274-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-274 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-274 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
arachidonate 5-lipoxygenase, RP11-67C2.3, ALOX5, ALOX5, 5-LO, 5LPG, LOG5 , arachidonic acid 5-lipoxygenase, leukotriene A4 synthase,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85kDa band observed in Human Bone Marrow and Rat Stomach lysates (calculated MW of 77.7.0kDa according to rat NP_036954). Recommended concentration: 0.3-1ug/ml.
Background References:
  • Kalayci O, Birben E, Sackesen C, Keskin O, Tahan F, Wechsler ME, Civelek E, Soyer OU, Adalioglu G, Tuncer A, Israel E, Lilly C. ALOX5 promoter genotype, asthma severity and LTC production by eosinophils. Allergy. 2006 Jan;61(1):97-103.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-ERNKKKQLPYYYLSPD
NCBI Gene ID #:
240
NCBI Official Name:
arachidonate 5-lipoxygenase
NCBI Official Symbol:
ALOX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000689.1
Protein GI Number:
4502057
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P09917