Arrestin Antibody

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ProSci
Product Code: PSI-45-295
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-295-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-295 (0.1ug/ml) staining of Human Spleen (A), (1ug/ml) Mouse Brain (B) and (1ug/ml) Mouse Spleen (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-295 (0.1ug/ml) staining of Human Spleen (A), (1ug/ml) Mouse Brain (B) and (1ug/ml) Mouse Spleen (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARRB2, arrestin, beta 2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2, arrestin 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Spleen, Mouse Brain lysates and in preliminary testing of HEK293 cell lysate, and approx 45kDa in Mouse Spleen lysates and in preliminary testing of A549 cell lysate (calculated MW of 48kDa according to Human NP_001244257.1, 46.1kDa according to Human NP_004304.1; 46.3kDa according to Mouse NP_001258289.1 and 47.4kDa according to Mouse NP_001258287.1 ) Recommended concentration: 0.1-1?g/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Lefkowitz RJ, Shenoy SK. Transduction of receptor signals by beta-arrestins. Science. 2005 Apr 22;308(5721):512-7. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-HDHIPLPRPQS
NCBI Gene ID #:
409
NCBI Official Name:
arrestin, beta 2
NCBI Official Symbol:
ARRB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004304.1, NP_945355.1, NP_001244257.1, NP_001244259.1, NP_001244260.1
Protein GI Number:
4757780
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms 1, 2, 3, 5 and 6 (NP_004304.1; NP_945355.1; NP_001244257.1; NP_001244259.1; NP_001244260.1). No crossreactivity is expected with Arrestin beta 1.
Swissprot #:
P32121