Centromere Protein E Antibody

ProSci
Product Code: PSI-45-392
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-392-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
CENPE, CENP-E, centromere protein E (312kD), Centromere autoantigen E (312kD), centromere protein E, 312kDa, KIF10, centromere protein E, kinesin family member 10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 316kDa according to NP_001804. Therefore we cannot recommend an optimal concentration and the product is Investigative Grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 1991 May;10(5):1245-54.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: HASSGKDVPECKTQ
NCBI Gene ID #:
1062
NCBI Official Name:
centromere protein E, 312kDa
NCBI Official Symbol:
CENPE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001804.2
Protein GI Number:
71061468
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q02224