CST8 Antibody

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ProSci
Product Code: PSI-42-303
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-303-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed with 42-303 (0.3ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with EB09823 (1mg/ml) in Lane
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed with 42-303 (0.3ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed with EB09823 (1mg/ml) in Lane

Documents

Further Information

Additional Names:
CST8, cystatin 8 (cystatin-related epididymal specific), CRES, cystatin 8, cystatin-related epididymal spermatogenic protein, cystatin-related epididymal-specific
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected HEK293 transiently expressing Human CST8 (myc and DYKDDDDK tagged), a band of approx. 23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 0.1-0.3ug/ml.Purchase the +ve control! The transfected lysate used in QC is available for sale. Please email sales@everestiotech.com to request datasheet, pricing and delivery information.
Background References:
  • Chapuis J, Hot D, Hansmannel F, Kerdraon O, Ferreira S, Hubans C, Maurage CA, Huot L, Bensemain F, Laumet G, Ayral AM, Fievet N, Hauw JJ, DeKosky ST, Lemoine Y, Iwatsubo T, Wavrant-Devri?ze F, Dartigues JF, Tzourio C, Buee L, Pasquier F, Berr C, Mann D, Lendon C, Alperovitch A, Kamboh MI, Amouyel P, Lambert JC. Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. Molecular psychiatry 2009 Nov 14 (11): 1004-16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-DPKKNETGVLRK
NCBI Gene ID #:
10047
NCBI Official Name:
cystatin 8 (cystatin-related epididymal specific)
NCBI Official Symbol:
CST8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005483.1
Protein GI Number:
4885163
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Swissprot #:
O60676