FOXC2 Antibody

ProSci
Product Code: PSI-45-605
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-605-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-605 staining (2ug/ml) of nuclear HEK293 (A) and (1ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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45-605 staining (2ug/ml) of nuclear HEK293 (A) and (1ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Further Information

Additional Names:
FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in lysates of cell lines K562 and HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Brice G, Mansour S, Bell R, Collin JR, Child AH, Brady AF, Sarfarazi M, Burnand KG, Jeffery S, Mortimer P, Murday VA. Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24. J Med Genet. 2002 Jul;39(7):478-83.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: RHAAPYSYDCTKY
NCBI Gene ID #:
2303
NCBI Official Name:
forkhead box C2 (MFH-1, mesenchyme forkhead 1)
NCBI Official Symbol:
FOXC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005242.1
Protein GI Number:
4885237
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q99958