FOXO4 Antibody

ProSci
Product Code: PSI-45-623
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-623-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-623 (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-623 (1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
MLLT7, FOXO4, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) translocated to, 7, AFX, AFX1, MGC120490 , myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) translocated to, 7
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60-65kDa band observed in lysates of cell line K562 (calculated MW of 53.7kDa according to NP_005929.2). Recommended concentration: 1-3ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml
Background References:
  • Paik JH, Kollipara R, Chu G, Ji H, Xiao Y, Ding Z, Miao L, Tothova Z, Horner JW, Carrasco DR, Jiang S, Gilliland DG, Chin L, Wong WH, Castrillon DH, DePinho RA. FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis. Cell. 2007 Jan 26;128(2):309-23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EAASQDRMPQDLD
NCBI Gene ID #:
4303
NCBI Official Name:
forkhead box O4
NCBI Official Symbol:
FOXO4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005929.2
Protein GI Number:
103472003
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P98177