MDFI Antibody

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ProSci
Product Code: PSI-42-165
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-165-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-165 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-165 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000016375, OTTHUMP00000196414, inhibitor of MyoD family a
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Lung (Bronchus). Recommended concentration: 5ug/ml.
Background References:
  • Ma R, Rundle D, Jacks J, Koch M, Downs T, Tsiokas L, Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1. The Journal of biological chemistry 2003 Dec 278 (52): 52763-72.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SKSTTSQIPLQAQED
NCBI Gene ID #:
4188
NCBI Official Name:
MyoD family inhibitor
NCBI Official Symbol:
MDFI
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005577.1
Protein GI Number:
5031905
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription
Swissprot #:
Q99750