Ribosomal protein L8 Antibody

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ProSci
Product Code: PSI-46-297
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-297-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 3
46-297 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
2 / 3
46-297 (2.5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
3 / 3
46-297 (0.01ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-297 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-297 (2.5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-297 (0.01ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ribosomal protein L8, 60S ribosomal protein L8
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28-30kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0kDa according to NP_000964.1 and NP_150644.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Cerebral Cortex and Spleen. Recommended concentration: 2.5ug/ml.
Background References:
  • Swoboda RK, Somasundaram R, Caputo L, Ochoa EM, Gimotty PA, Marincola FM, Van Belle P, Barth S, Elder D, Guerry D, Czerniecki B, Schuchter L, Vonderheide RH, Herlyn D. Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display. Cancer Res. 2007 Apr 15;67(8):3555-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: KAGRAYHKYKAKRNC
NCBI Gene ID #:
6132
NCBI Official Name:
ribosomal protein L8
NCBI Official Symbol:
RPL8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000964.1
Protein GI Number:
4506663
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
Swissprot #:
P62917