Tribbles Homolog 1 Antibody

ProSci
Product Code: PSI-46-912
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-912-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-912 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-912 (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
TRIB1 , tribbles homolog 1 (Drosophila) , C8FW, GIG2N: SKIP1, G-protein-coupled receptor induced protein, phosphoprotein regulated by mitogenic pathways
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Lymph Node and Peripheral Blood Mononucleocyte lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.0kDa according to NP_079471.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Background References:
  • Sung HY, Guan H, Czibula A, King AR, Eder K, Heath E, Suvarna SK, Dower SK, Wilson AG, Francis SE, Crossman DC, Kiss-Toth E. Human tribbles-1 controls proliferation and chemotaxis of smooth muscle cells via MAPK signaling pathways. J Biol Chem. 2007 Jun 22;282(25):18379-87. Epub 2007 Apr 23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cow
Immunogen:
The immunogen for this antibody is: C-TSDQIVPEYQEDSD
NCBI Gene ID #:
10221
NCBI Official Name:
tribbles pseudokinase 1
NCBI Official Symbol:
TRIB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079471.1
Protein GI Number:
13399328
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Stem Cell
Swissprot #:
Q96RU8