UPF3A Antibody
| Code | Size | Price |
|---|
| PSI-42-211-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
UPF3A, UPF3 regulator of nonsense transcripts homolog A (yeast), HUPF3A, RENT3A, UPF3, OTTHUMP00000018789, UPF3 regulator of nonsense transcripts homolog A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of cell lines HeLa and Kelly after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 51kDa according to NP_542418.1 and the reported sizes of 65 to 70kDa from different sources. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Tarpey PS, Raymond FL, Nguyen LS, Rodriguez J, Hackett A, Vandeleur L, Smith R, Shoubridge C, Edkins S, Stevens C, O'Meara S, Tofts C, Barthorpe S, Buck G, Cole J, Halliday K, Hills K, Jones D, Mironenko T et al. Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation. Nature genetics 2007 Sep 39 (9): 1127-33.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Cow
Immunogen:
The immunogen for this antibody is: C-KETDKQKKIAEKE
NCBI Gene ID #:
65110
NCBI Official Name:
UPF3 regulator of nonsense transcripts homolog A (yeast)
NCBI Official Symbol:
UPF3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_075387.1, NP_542418.1
Protein GI Number:
12711676
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
Swissprot #:
Q9H1J1