NQO1 Antibody

Product Code: PSI-45-981

Supplier: ProSci

Code	Size	Price

PSI-45-981-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Canine (dog)
Human
Mouse

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-981 (1ug/ml) staining of Rat (A) and Pig (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

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45-981 staining (0.1ug/ml) of U251 cell lysate (RIPA buffer, 35ug total protein per lane). Detected using chemiluminescence.

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45-981 staining (0.03ug/ml) of Human Kidney (A) and (0.3ug/ml) Lung (B) lysate (RIPA buffer, [35ug total protein per lane). Detected using chemiluminescence.

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45-981 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

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Documents

Further Information

Additional Names:

NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1

Application Note:

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in lysates of Glioblastoma cell line U251 and approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 27.3kDa according to Human NP_001020604.1 and 30.9kDa according to Human NP_000894.1, Rat NP_058696.2 and Pig NP_001153085.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and U251 cells. Recommended concentration: 5-10ug/ml.

Background References:

Asher G, Lotem J, Kama R, Sachs L, Shaul Y. NQO1 stabilizes p53 through a distinct pathway. Proc Natl Acad Sci USA. 2002 Mar 5;99(5):3099-104.

Buffer:

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.

Concentration:

500 ug/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Expected Species Reactivity based on sequence homology: Dog

Immunogen:

The immunogen for this antibody is: C-SIPTDNQIKARK

NCBI Gene ID #:

1728

NCBI Official Name:

NAD(P)H dehydrogenase, quinone 1

NCBI Official Symbol:

NQO1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_000894.1, NP_001020604.1, NP_001020605.1

Protein GI Number:

4505415

Purification:

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Research Area:

Cancer

SPECIFICITY:

This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

Swissprot #:

P15559