CHFR Antibody
| Code | Size | Price |
|---|
| PSI-25-840-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHFR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
CHFR, FLJ10796, FLJ33629, RNF116, RNF196
Application Note:
CHFR antibody can be used for detection of CHFR by ELISA at 1:312500. CHFR antibody can be used for detection of CHFR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Background References:
- Kang, H.C., Cancer Lett. 260 (1-2), 170-179 (2008).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHFR.
NCBI Gene ID #:
55743
NCBI Official Name:
checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
NCBI Official Symbol:
CHFR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
NP_060693
Protein GI Number:
239048907
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cancer ,Apoptosis ,Homeostasis
Swissprot #:
Q96EP1
User NOte:
Optimal dilutions for each application to be determined by the researcher.