IL-8 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5199Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-8 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8
Application Note:
ELISA:
Sandwich:
To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-8 (XP-5199) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-8 (XP-5199) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8).
NCBI Gene ID #:
3576
NCBI Official Name:
interleukin 8
NCBI Official Symbol:
IL8
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10145
Protein GI Number:
124359
Purification:
Anti-hIL-8 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P10145
User NOte:
Centrifuge vial prior to opening.