GDF-3 Antibody

ProSci
Product Code: PSI-38-156
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-156-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
GDF-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

1 / 4
To detect hGDF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GDF-3 (38-157) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDF-3.
2 / 4
3 / 4
4 / 4
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

To detect hGDF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GDF-3 (38-157) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDF-3.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Further Information

Additional Names:
KFS3, MCOP7, MCOPCB6GDF-3
Application Note:
ELISA:
Indirect:
To detect hGDF-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDF-3.

Sandwich
To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human GDF-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDF-3.

Western Blot:
To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDF-3. Human GDF-3 specific antibody was purified by affinity chromatography employing immobilized hGDF-3 matrix.
NCBI Gene ID #:
9573
NCBI Official Name:
growth differentiation factor 3
NCBI Official Symbol:
GDF3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9NR23
Protein GI Number:
46397885
Research Area:
Signal Transduction,Growth Factor,Antibody Pairs
Swissprot #:
Q9NR23
User NOte:
Centrifuge vial prior to opening.