PlGF Antibody
| Code | Size | Price |
|---|
| PSI-38-151-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PlGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
PGFL, PLGF, PlGF-2, D12S1900, SHGC-10760, PGFL, Placenta growth factorPlGF
Application Note:
ELISA:
Direct:
To detect hPlGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Sandwich
To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PlGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Western Blot:
To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Direct:
To detect hPlGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Sandwich
To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human PlGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPlGF.
Western Blot:
To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
PLGF (also called Placenta Growth Factor) is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPlGF. Human PlGF specific antibody was purified by affinity chromatography employing immobilized hPlGF matrix.
NCBI Gene ID #:
5228
NCBI Official Name:
placental growth factor
NCBI Official Symbol:
PGF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P49763
Protein GI Number:
17380553
Research Area:
Antibody Pairs
Swissprot #:
P49763
User NOte:
Centrifuge vial prior to opening.