WNT-3a Antibody
| Code | Size | Price |
|---|
| PSI-38-177-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
WNT-3a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
Protein Wnt-3a
Application Note:
ELISA:
Indirect:
To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect:
To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human WNT-3a as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized hWNT-3a matrix.
NCBI Gene ID #:
89780
NCBI Official Name:
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol:
WNT3A
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P56704
Protein GI Number:
20532424
Research Area:
Stem Cell,Neuroscience,Antibody Pairs
Swissprot #:
P56704
User NOte:
Centrifuge vial prior to opening.