ProSci

IBSP Antibody

Product Code:
 
PSI-28-550
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IBSP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
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Antibody used in WB on Human HepG2 at 1.25 ug/ml.

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PSI-28-550-100ul100ul£609.00
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Further Information

Additional Names:
IBSP, BSP, BNSP, SP-II, BSP-II
Application Note:
IBSP antibody can be used for detection of IBSP by ELISA at 1:62500. IBSP antibody can be used for detection of IBSP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
Background References:
  • Barnes, G.L., et al., (2003) Cancer Res. 63 (10), 2631-2637.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IBSP.
NCBI Gene ID #:
3381
NCBI Official Name:
integrin-binding sialoprotein
NCBI Official Symbol:
IBSP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
NP_004958
Protein GI Number:
167466187
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cancer ,Signal Transduction
Swissprot #:
P21815
User NOte:
Optimal dilutions for each application to be determined by the researcher.